The Disease Model Core offers a wide range of support to researchers at the MDU and the Institute of Metabolic Science. The Core offers a range of specialised techniques available to both researchers within the institute and academic and external collaborators. The services offered include, but are not limited to, husbandry, breeding transgenic lines and general phenotyping.
We also work with investigators to develop the range of procedures we offer.
The DMC offers access to equipment, assistance and training for:
- Indirect calorimetry studies
- Temperature controlled conditions for housing and experimental procedures
- Body composition analysis
- Food and water consumption
- Body temperatures
- Advanced surgical procedures
- Stable isotope clamping and telemetry as well as more standard procedures within preclinical metabolic research in mice (for example: Glucose tolerance tests, Insulin tolerance tests etc)
The DMC will guide and assist you through the required steps for initiating your in vivo studies – in the first instance, please contact the DMC
DMC Equipment:
- Sable Systems Promethion core
- Sable Systems Stable Isotope Gas Analyser
- Zinsser Analytical EchoMRI 100H
- DSI PhysioTel HD Telemetry System
- TSE Stellar Telemetry for Mice
- MemmertTM Constant Climate Chamber HPP750
- MedAssociates Operant Chambers and Food Intake Analysis Software
- IKA C1 Bomb Calorimeter
- Hidex 300SL Scintillation Counter
- Flir T540 Therma Camera
Cambridge Biomedical Campus University Facilities:
- Surgery suite (including pre and post-op)
- Perfusion room
- Sch1 room
- ECHO cardiography
- CL2 & 3 specific areas
- Irradiator
- IVIS® Spectrum Imaging System
- mPET/CT
- MRI
- Multi-photon imaging
- Ultrasound
- Echocardiography
Recent publications supporting the DMC
- Mann JP, Duan X, Patel S, Tábara LC, Scurria F, Alvarez-Guaita A, Haider A, Luijten I, Page M, Protasoni M, Lim K, Virtue S, O’Rahilly S, Armstrong M, Prudent J, Semple RK, Savage DB. A mouse model of human mitofusin 2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion. Elife. 2023 Feb 1;12:e82283. doi: 10.7554/eLife.82283. PMID: 36722855
- Buller S, Kohnke S, Hansford R, Shimizu T, Richardson WD, Blouet C. Median eminence myelin continuously turns over in adult mice. Mol Metab. 2023 Feb 3;101690. doi: 10.1016/j.molmet.2023.101690. PMID: 36739968
- Patel S, Haider A, Alvarez-Guaita A, Bidault G, El-Sayed Moustafa JS, Guiu-Jurado E, Tadross JA, Warner J, Harrison J, Virtue S, Scurria F, Zvetkova I, Blüher M, Small KS, O’Rahilly S, Savage DB. Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice. Mol Metab. 2022 Nov;65:101589. doi: 10.1016/j.molmet.2022.101589. PMID: 36064109
- Lewis JE, Woodward OR, Nuzzaci D, Smith CA, Adriaenssens AE, Billing L, Brighton C, Phillips BU, Tadross JA, Kinston SJ, Ciabatti E, Göttgens B, Tripodi M, Hornigold D, Baker D, Gribble FM, Reimann F. Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons modulate food intake and preference in mice. Mol Metab. 2022 Sep 30:101604. doi: 10.1016/j.molmet.2022.101604. PMID: 36184065
- Pellegrinelli V, Rodriguez-Cuenca S, Rouault C, Figueroa-Juarez E, Schilbert H, Virtue S, Moreno-Navarrete JM, Bidault G, Vázquez-Borrego MC, Dias AR, Pucker B, Dale M, Campbell M, Carobbio S, Lin YH, Vacca M, Aron-Wisnewsky J, Mora S, Masiero MM, Emmanouilidou A, Mukhopadhyay S, Dougan G, den Hoed M, Loos RJF, Fernández-Real JM, Chiarugi D, Clément K, Vidal-Puig A. Dysregulation of macrophage PEPD in obesity determines adipose tissue fibro-inflammation and insulin resistance. Nat Metab. 2022 Apr;4(4):476-494. doi: 10.1038/s42255-022-00561-5. Epub 2022 Apr 25. PMID: 35478031
