Research focus
My research project involves generating and analysing large datasets of single-cell and bulk RNA sequencing data. The results of this analysis then informs the development of mammalian intestinal organoid models, which help us to investigate how specific enteroendocrine cell types respond to certain nutrients.
Background and experience
As a maths and physics graduate, I directed myself into a biological setting by doing my PhD at the University of Warwick in a department aimed at the intersection between the sciences. This allowed me to develop my lab-based, microscopy and computational experience in the field of cell division. Since moving to the IMS five years ago, I have expanded on this experience by continuing to use microscopy to monitor cell activity, while also learning and developing our gut organoids and RNA sequencing platforms.
Working at the IMS-MRL
In particular, I am involved in:
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Handling RNA sequencing data from start to finish, including sample prep, read alignment to the genome, right through to detailed analysis of combined datasets,
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Generation and development of human and mouse organoids from the small and large intestines, including CRISPR knock-in to label specific types of enteroendocrine cells,
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Widefield fluorescence imaging to measure levels of intra-cellular calcium or cyclic AMP upon external stimuli, using single wavelength or FRET-based fluorescent proteins.
