The Tissue and Cell Imaging Core (TCI) provides access to high resolution and high content light microscopy, including live cell fluorescence imaging, and immunofluorescence-labelled or immunohistochemically-stained tissue on slides. Multiple software packages are also available for downstream analysis of acquired images.
The Core offers training, access and support in advanced light microscopy and downstream image analysis, including laser scanning confocal microscopy, high-throughput/high-content imaging of tissue slices and live cell cultures, and AI-driven image analysis software. We work closely with investigators and our other core facilities to develop and provide a streamlined workflow for users.
Services provided
- Introductory training on all microscopes and analysis workstations
- Workshops for analysis packages available within the Core
- Support in planning of experiments to ensure optimal results
- Maintained stock of imaging plates for purchase for high content imaging
- Provide contact with neighbouring imaging facilities, in the situation the Core does not have the equipment available to answer a given research question
- Regularly work with users and investigators to develop the equipment and abilities of the Core
For access or further support on any of the Core’s equipment, the TCI Core Manager will be very happy to discuss.
Equipment available
Light microscopes:
Perkin Elmer Opera Phenix (high content imaging with environmental chamber)
Zeiss Axio Scan Z1 (slide scanner)
Leica SP8 (scanning confocal with WLL and environmental chamber)
Image analysis workstations:
Perkin Elmer Harmony analysis workstation:
Indica Labs Halo analysis workstations (with HALO AI):
Imaris 10.2 analysis workstation
Key publications
Thomas L. Williams, Maria T. Colzani, Robyn G. C. Macrae, Emma L. Robinson, Stuart Bloor, Edward J. D. Greenwood, Jun Ru Zhan, Gregory Strachan, Rhoda E. Kuc, Duuamene Nyimanu, Janet J. Maguire, Paul J. Lehner, Sanjay Sinha, Anthony P. Davenport (2021) Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection. Commun Biol. 2021a; 4: 926. Published online 2021 Jul 29. doi: 10.1038/s42003-021-02453-y PMID: 34326460 PMCID: PMC8322398
Thomas L. Williams, Gregory Strachan, Robyn G. C. Macrae, Rhoda E. Kuc, Duuamene Nyimanu, Anna L. Paterson, Sanjay Sinha, Janet J. Maguire, Anthony P. Davenport (2021) Differential expression in humans of the viral entry receptor ACE2 compared with the short deltaACE2 isoform lacking SARS-CoV-2 binding sites. Sci Rep. 2021b; 11: 24336. Published online 2021 Dec 21. doi: 10.1038/s41598-021-03731-9 PMID: 34934117 PMCID: PMC8692523
